Riccardo Angelini
Company
Plant Biochemistry and Plant Physiology
Address
University “Roma Tre”
Faculty of Physical, Mathematical and Life Science, Department of Biology, Viale Guglielmo Marconi 446
00146 Rome
Telephone
+39-0657336361
E-mail
angelini@uniroma3.it
1. Summary of work to be allocated into a specific WG
This partner will perform integrated functional genomics, included RNAi strategy, and biological analyses of genes involved in polyamine catabolism, namely copper amine oxidases (CuAO) and flavin polyamine oxidases (PAO) in maize, tobacco and arabidopsis to find correlations between specific functions and drought, salt and cold tolerance. The work is mostly integrated into WG4.
2. Links with other WG and/or Subgroups
Not established yet. Activities can be potentially linked with other WG in order to study interactions with other signaling/metabolic pathways.
3. Specific activities to be integrated into WG and/or Subgroubs.
4. Qualification for the role and current grants
Riccardo Angelini, Full Professor of Plant Biochemistry at the Biology Department, University "Roma Tre", is considered as one the leading experts on the biochemistry, physiology and molecular genetics of plant amine oxidases. Different aspects of plant amine oxidases have been studied by Prof. Angelini in the last 20 years: A) cloning CuAO and PAO genes; B) CuAO and PAO regulation in development and defence; C) three-dimensional structure of maize PAO; D) ectopic expression of CuAO and PAO in tobacco and arabidopsis. Of particular relevance are the results obtained by the proponent on the determination of the first ever-known primary and tertiary structure of a flavin polyamine oxidase. Grants available from Ministry of University and Research and other sources for financing the research activities (including personnel costs) related to this COST Action.
5. Technical expertise and facilities
This partner will provide all of the expertise required for HPLC determination of polyamines, determination of polyamine catabolic enzyme activities, and also expertise on molecular biological, histological and immunocytochemical techniques applied to plant tissues and cells, including TEM-immunocytochemistry, gene cloning and biochemical characterization of recombinant proteins, plant transformation, etc. The laboratory has all the expertise and facilities to carry out the work allocated. In particular walk-in growth chambers, HPLC, spectrophotometers, centrifuges, optical and confocal microscopes, TEM.